picasso_addon: Picasso extensions

This package provides some further functionalities based on picasso python package including:

• Automated minimal net-gradient detection for picasso.localize
• Automated cluster detection (picks) based on picasso.render
• Easy to use script batch processing

We provide other packages that build up on picasso_addon:

• SPT : Complete single particle tracking analysis package (mobile and immobile)
• lbFCS: For molecular counting and hybridization rate measurements in localization clusters based on DNA-PAINT.

picasso_addon was used for data analysis in:

• Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
• Toward Absolute Molecular Numbers in DNA-PAINT
• Tracking Single Particles for Hours via Continuous DNA-mediated Fluorophore Exchange

Contents

• Installation
  • Create conda environment
• Usage
  • Localize and undrift
  • Autopick
  • Detailed info
• API
  • Submodules
• Contact